Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAKLEVKDNKKLVLKSVICKKLHDTK--VEDVDQEINRFHQHLQL-LKAQIFGPLIVKSCGTTIHDDGLITTDFEFYIQAHNAQQYSNIYDVQDSISVPYCLYVRFEDSPEYLQYAYSKLDLY-VYENDIQTD-GIVYTVYVNSS----PEKMVVDIFIPIVSL 5XQL Chain:A ((146-290)) ----------ERPAFSVVGMEYF-GSAP--TIGQLWERFIPREHEIAGKHDPEVSYGICAQQP-----NGEFHYVAGFEVQEGWPVPEGMVRFQVPA-QKYAVFTHKGTAPQIAESFQAIYSHLLAERGLEPKAGVDFEYYDQRFRGPLDPNSQVDLYIPIY--

General information: TITO was launched using: RESULT: Template: 5XQL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 516 4142 8.03 30.91 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 8.03 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.441

(partial model without unconserved sides chains): PDB file : Tito_5XQL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5XQL-query.scw PDB file : Tito_Scwrl_5XQL.pdb: