Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKMERNINMKKWQTCVLGAGSLLCLTACSGKSVTSEHQTKDEMKTEQTASKTSA-AKGKEVADFELMGVDGKTYRLSDYKGKKVYLKFWASWCSICLASLPDTDEIAKEAGDDYVVLTVVSPGHKGEQSEADFKNWYKGLDYKNLPVLVDPSGKLLETYGVRSYPTQAFIDKEGKLVKTHPGFMEKDAILQTLKELS 2YP6 Chain:C ((43-185)) -------------------------------------------------------IAVGKDAPDFTLQSMDGKEVKLSDFKGKKVYLKFWASWCGPCKKSMPELMELAAKPDRDFEILTVIAPGIQGEKTVEQFPQWFQEQGYKDIPVLYDTKATTFQAYQIRSIPTEYLIDSQGKIGKIQFGAISNADAEAAFKEMN

General information: TITO was launched using: RESULT: Template: 2YP6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 666 15376 23.09 108.28 target 2D structure prediction score : 0.77 Monomeric hydrophicity matching model chain C : 0.76 3D Compatibility (PKB) : 23.09 2D Compatibility (Sec. Struct. Predict.) : 0.77 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.792

(partial model without unconserved sides chains): PDB file : Tito_2YP6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2YP6-query.scw PDB file : Tito_Scwrl_2YP6.pdb: