Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKADTIFKENIERILKEGVFSEQARPKYKDGTVANSKYVTGAFSEYDLSKGEFPITTLRPIAIKSAIKEVLWIYQDQSNSLEVLNDKYNVHYWNDWEVGDTGTIGERYG-------AVVKKH-DIINKLLKQLETNPWNRRNIISLWDYQAFEETDGLLPCAFQTMFDVRRVDGEIYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNLHIYDNQFEQAQELLRREPSNCQPRLVLNVPDGTNFFDIKAEDFELVDYDPVKPQLKFDLAI 4FOA Chain:C ((14-261)) ----------------------ETGTPK-SDRTGTGTRSLFGQQMRYDLSAG-FPLLTTKKVHFKSVAYELLWFLRGDSN-IGWLHE-HGVTIWDEW-ASDTGELGPIYGVQWRSWPAPSGEHIDQISAALDLLRTDPDSRRIIVSAWNVGEIERM-ALPPC--HAFFQFYVADGR--LSCQLYQRSADLFLGVPFNIASYALLTHMMAAQAGLSVGEFIWTGGDCHIYDNHVEQVRLQLSREP-RPYPKLLLA--DRDSIFEYTYEDIVVKNYDP-HPAIKAP---

General information: TITO was launched using: RESULT: Template: 4FOA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1123 -22458 -20.00 -93.58 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain C : 0.74 3D Compatibility (PKB) : -20.00 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.530

(partial model without unconserved sides chains): PDB file : Tito_4FOA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4FOA-query.scw PDB file : Tito_Scwrl_4FOA.pdb: