Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---MEFFICNLVRVVQSPRFYMSLFLTLLCMSLGNFLAFNGIYKIEGLSIFFAASSIR-------------GFSPISLVAALICTL----------PYSSQI-IEDAESHFLTAQLCRI---SKKRYLA-IVGSTAIISSFLVFFLPYLLL-LGI-NLLVTPYQEIYIGDYSGALKELFDSNQLLYSL-VTTLWYGVWGAVFSIFGLASALLVKKKLGAIFIPVAYMMVG-GI-LWAILGLSYLEPVTTLAL-GYQKDISLSLVSAHLAFIL---FVSCLVVYGTFF-LHSEDYV 3G3L Chain:A ((10-305)) PAEAKYYIAGTITDATTGQELTTAKVTLGDKSVTSSFNEQVNYKAEGYALVVSADGYYPVKRQVYLNQVSDGQTSVATVNVALVSVEAAVIPPVVPPTDPETDINEGEATKVADKAVEVAKPSESTVTDMLAGTTA--TPEEKKALDETLEMAGGMKVGETTPEVLADGSILAITPVKFTNPIQDAPAMVPYFYNEGCELTGDVKEVAAPVAVAADIQKAFLSNAAKALNMNAGFVQKIGYTRISVLNGYSILGYT--IKGQLVSKKLTFLISGKYYEGIVSYQKSVMIYPNYYS

General information: TITO was launched using: RESULT: Template: 3G3L.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1091 -67462 -61.84 -269.85 target 2D structure prediction score : 0.30 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -61.84 2D Compatibility (Sec. Struct. Predict.) : 0.30 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.144

(partial model without unconserved sides chains): PDB file : Tito_3G3L.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3G3L-query.scw PDB file : Tito_Scwrl_3G3L.pdb: