Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -MSRLLVIGCGGVAQVAISKICQDSETFTEIMIASRTKSKCDDLKAKLEGKTSTKIETAALDADKVEEVIALIGSYKPEAVLNVALPYQDLTIMDACLATGVHYIDTANYEAEDTEDPEWRAIYEKRCKELGFTAYFDYSWQWAYQEKFKEAGLTALLGSGFDPGVTSVFSAY-ALKHYFDEIHYIDILDCNGGDHGYPFATNFNPEINLREVSAPGSYWEDGKWVEVEAMSIKREYDFPQVGQKDMYLLHH-E-EIESLAKNIPGVKRIRF-FMTFGQSYLTH-MKCLENVGLLRTDTINFNGQEIVPIQFLKALLPDPASLGPRTVGKTNIGCIFTGVK--DGVEKTIY-IYNVC-DHQECYAEVGSQA-ISY-TTGVPAMIGTKLVMNGT---WKQAGVYNLEELDPDPFMEALNEYGLPWVVVENPQMVD 3BIO Chain:A ((8-304)) KKIRAAIVGYGNIGRYALQALREAPD----FEIAGIVRR----------------------ELQPFRVVSDIEQLESVDVALVCSPSREVERTALEILKKGICTADS---FDIHDGILALRRSLGDAAGKSG---------------------AAAVIASGWDPGSDSVVRTLMQ--------------------------------------------------------------AIVPKGITYTNFGPGMSMGHTVAVKAIDGVKAALSMTIPLGTGVHRRMVYVELLPG------------------------HNLEEVSAAIKADEYFVHDETHVIQVDEVDALIDMGHGVRMVRKGVSGSTQNQRMSFDMEINNPALTGQVLVCAARAAMRQQPGAYTLQEIPVIDLLPGDREQWIGKLC--------

General information: TITO was launched using: RESULT: Template: 3BIO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1538 9020 5.86 32.80 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : 5.86 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.290

(partial model without unconserved sides chains): PDB file : Tito_3BIO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3BIO-query.scw PDB file : Tito_Scwrl_3BIO.pdb: