Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----MFVARDARGELVNVLED-----------------KLEKQAYTCPACGGQLHLRQGPSVRTHFAHKSLKDCDFFFENESPEH-LANKESLYHWL-KKETKVQLEYPLSELKQIADVFVNGNL-------------------ALEVQCSPLPQKVLKERSEGYRSQGYQVL-WLLGQKLWL-----KERLTRLQQGFLYFSQNMGFYVW-ELD-KGKQVLRLKYLI-----YQDLRGKLHYQIKEFSYGQGSLLEILRLPYKKQKISHFTVSE-DKDI---CRYIRQQLYYQNLFWMKEQAEAYQKGENILTYGLKEWYPQIRP--------------IVGKFFQI----EQDLTSYYQHFYTYYQKNPQNDWQ-KLYPPAFY-Q------QYFLKNMVE--------------------------------------------------------------- 4OWT Chain:A ((33-498)) GGRLLLSTSLDAKDELEERLERCMSIVTSMTAGVSEREANDALNAYVCKGLPQHEEICLGLFTLILTEPAQAQKCYRDLALVSRDGMNIVLNKINQILMEKYLKLQDTCRTQLVWLVRELVKSGVLGADGVCMTFMKQIAGGDVTAKNIWLAESVLDILTEQREWVLKSSILIAMAVYTYLRLIVDHHGTAQLQALRQKEVDFCISLLRERFMECLMIGRDLVRLLQNVARIPEFELLWKDIIHNPQALSPQFTGILQLLQSRTSRKFLACRLTPDMETKLLFMTSRVRFGQQKRYQDWFQRQYLSTPDSQSLRCDLIRYICGVVHPSNEVLSSDILPRWAIIGWLLTTCTSNVAASNAKLALFYDWLFFSPDKDSIMNIEPAILVMHHSMKPHPAITATLLDFMCRIIPNFYPPLEGHVRQGVFSSLNHIVEKRVLAHLAPLFDNPKLDKELRAMLREKFPEFCS

General information: TITO was launched using: RESULT: Template: 4OWT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 3 -406 -135.33 -1.28 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : -135.33 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.58 QMean score : ?

(partial model without unconserved sides chains): PDB file : Tito_4OWT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4OWT-query.scw PDB file : Tito_Scwrl_4OWT.pdb: