Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRKQIHPLILFSFFGIMMFILIINRPLNDSQSFKTKSNIAQIEAQALKHLDKPIIDLSGWQRPEEINYDALSQ-NISGAIVRVHSGTQTTKENDASFINGIDKAYKSHITELQKRNVPVAVYAYVAGKSVQEMEKAAEVFYNAASPYSPSY----YWLDVE---------DKTMSNMNEGVENFRAKLASLGAKNIGIYVGVYFMEEHSIDTGKFTS---VWIPSYGSDSGFLESSPKTDLDYDIHQYTSKGKIAGFDHDLDINVISPLKNKEETFRKLFLKP- 1JFX Chain:A ((1-217)) -----------------------------------------------DTSGVQGIDVSHWQGS--INWSSVKSAGMSFAYIKATEGTNYK-----------DDRFSANYTNAYNAGIIRGAYHFARPNA-SSGTAQADYFASNGGGWSRDNRTLPGVLDIEHNPSGAMCYGLSTTQMRTWINDFHARYKARTTRDVVIYTTASWWNTCTGSWNGMAAKSPFWVAHWGVSAP---TVPSGFPTWTFWQYSATGRVGGVSGDVDRNKFN---GSAARLLALANNTA

General information: TITO was launched using: RESULT: Template: 1JFX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1103 13301 12.06 66.84 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 12.06 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.475

(partial model without unconserved sides chains): PDB file : Tito_1JFX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1JFX-query.scw PDB file : Tito_Scwrl_1JFX.pdb: