Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----MKQERFPLVSDDEVMLTEMPVMNLYDESDLISNIKGEYRDKNYLEWAPIAEEKPVKPIEKQVEKPKKAPLGVKKEGKSYAEVAREEARADLKK--KRSANYLTQDFSLARRHSQPSLVRQGNQPTTPFQKENPGEFVKYSQKLTQSHYILAEEVHSIPTKNEE---VSAPAPKKNNYDFLKKSQIYNKKSKQTEQERRVAQELNLTRMTE 1EEX Chain:B ((46-223)) GFLTEVGEARQGTQQDEVIIAVGPAFGLA----QTVNIVGIPHKSILREVIAGIEEEGI-----------------------KARVIRCFKSSDVAFVAVEGNRLSGSGISIGIQSKGTTVIHQQGLP-----PLSNLELFPQAPLLTLETYRQIGKNAARYAKRESPQPVPTLNDQMARPKYQAKSAILHIKETKYVVTGKNPQELRVA----

General information: TITO was launched using: RESULT: Template: 1EEX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 931 45141 48.49 267.10 target 2D structure prediction score : 0.39 Monomeric hydrophicity matching model chain B : 0.65 3D Compatibility (PKB) : 48.49 2D Compatibility (Sec. Struct. Predict.) : 0.39 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.138

(partial model without unconserved sides chains): PDB file : Tito_1EEX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1EEX-query.scw PDB file : Tito_Scwrl_1EEX.pdb: