Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKNIRIGSYHWMQTTNGQLSFKEKIKLIQKIMVPSILSSIKINYYRFQTGNDFDIGQIVIPDTQMIKIALEELESKASISIYNHSWRTYFWGAALGH--LQNQQFDPESLLLASLFHDIGLTEQHMHSKGCQCFTYESAKQFEQKAKEYNFDDK-KSEVIKDAICLH-MNGYIEDSDPPEVV---LLQQGASCDVISDNQYKLPLSFRNKIL--EKYPRNQFNKEFIKLINLERKNVPNSRTGLLYDLGLPLMIKSNLYNENLI 5TPI Chain:A ((7-211)) QGLFSLGS----------------LESTAAVRIPSTLAHFNQRYPKIHLALSTG------PSGTMIDGVLEGALS------------AAFVDGPLVHPGLEGLPVFPEEMMIVAPYGHAPITRASEVN-GANVYAFRANCSYRRHFESWFHADRATPGRIHEMESYHGMLACVIAGAGLALIPRSMLESMPGHQQVSAWPLAEEWRWLTTWLVWRRGAKTRQLEAFIALLNEDRQTVVSP------------------------

General information: TITO was launched using: RESULT: Template: 5TPI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1023 -8565 -8.37 -43.70 target 2D structure prediction score : 0.39 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -8.37 2D Compatibility (Sec. Struct. Predict.) : 0.39 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.213

(partial model without unconserved sides chains): PDB file : Tito_5TPI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5TPI-query.scw PDB file : Tito_Scwrl_5TPI.pdb: