Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNTFVDAARSFRTQFDLNFSEKIIVDFFAGGGGASTGLEMGLNR-PVYVAVNHNPKAISMHEANHPHAKHYIQDVFAVDP------------IDICDGHQVGWFHASPDCTHHSQAAGGQPR--KKEIRDLSWVVLKFAGKVKPDVISLENVKQILGWGPLIAKRDKATGRVITLDKININGKKVNRIAEPGERVPRHNQFLVPNPKKKGKTWKHFVRSLEQLGYEVEWQKNIIAADFGAPTKRERLFLIARCDGQPI-VWPEKYFSKKPK---------------------GNLKKWRSTVECVDFSDLGNSIFDRPQGPLADATLKRIAKGIQKYVIETKEPFFVNSSTPFIGRDFRTSFGHDIREPLATTTAGYGG------HSSLISPILVPFITEFANASQQRNWSIDEPLSTICAQVKGGHHGLVTA---------------------------------------------------KLSKDNYKGALRVAAFLINYYGNGDARSITEPMDTITTKDRLVLVTVWIKGEPWAIVDICIRMLKPRELFRAQGVPDSYVIEYGSDGKPLSKKDQVFMVGNSVSPYPMAAIARANNPFITQQIKGAA 4DA4 Chain:A ((409-871)) -----------------KLPKLRTLDVFSGCGGLSEGFHQA-GISETLWAIEMWDPAAQAFRLNNPGTTVFTEDCNVLLKLVMAGEVTNSLGQRLPQKGDVEMLCGGPPCQGFSGMNRFNSRTYSKFKNSLVVSFLSYCDYYRPRFFLLENVRNFVSYRR-------------------------------------------------SMVLKLTLRCLVRMGYQCTFGV-LQAGQYGVAQTRRRAIILAAAPGEKLPLFPEPLHVFAPRACQLSVVVDDKKFVSNITRLSSGPFRTITVRDTMSDLPE-IQNGAS-NSEI-----------------------------------------PYNGEPLSWFQRQLRGSHYQPILRDHICKDMSPL---------VAARMRHIPLFPG------SDWRDLPNIQVRLGDGVIAHKLQYTFHDVKNGYSSTGALRGVCSCAEGKACDPESRQFSTLIPWCLPHTGNRHNHWAGLYG---RLEWDGFFSTTVTNPE-----PMGK-QGRVLHPEQHRVVSVRECARSQGFPDSYRFF------GN-ILDRHRQVGNAVPPPLAKAIGLEIKLCLLS------

General information: TITO was launched using: RESULT: Template: 4DA4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1599 7965 4.98 21.58 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : 4.98 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.293

(partial model without unconserved sides chains): PDB file : Tito_4DA4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4DA4-query.scw PDB file : Tito_Scwrl_4DA4.pdb: