Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------------------MHEITLHEVRQLIASLRTVYAAQFNKQFPTSGESAIPLSVVEQIA----LKTLVGVQQNQFNNALARLLTA-------------GGRF---MPSFAEFRTWCIGE------------SWMSPEEAWSRACKFTTDR---TVVI--TQITKYALDE------VMY----LIEAGQMRAAQDNFFGTYNVMV--AKAQLKGRQQEFYTPPLQLEHKEPEHTPVS--NDEVQKQLKSLMERL---KINGRKPAPVQKLQAKEKEPELAKELGP-DPFDNPHEYA-EMCRREGMPIPRNILQLIEGANV-------- 5AX7 Chain:A ((1-348)) KKPLFTKSPRNSASCESTITLQSNLLFTYYKHYFAGIKKVALIGFPDH-PNKGDSAIYVAEKKLLDALNIEVVYITAQEADYSASELKSIISDIPRDEFALAFHGGGNFGDLYPDHQHLRELVVRDFPSFTTISFPQSVWYNEQQLLEQASILYAENPNITLVTRDRQSYGFAVDAFGKHNEVLLTPDIVFFMGPIPEIREATPITHDVLILARLDTLNAANLTYSVEDWLLWDPPVAQNPDSSFDDRGQARYEAGAEFLASARVVITDRLHAHILSTLMGIPHIVVENSQMGKITNYHNTWLHGCTLDGVSVVVDSVDKALSLLLEWNEAGYF

General information: TITO was launched using: RESULT: Template: 5AX7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1055 20712 19.63 83.51 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 19.63 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.109

(partial model without unconserved sides chains): PDB file : Tito_5AX7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5AX7-query.scw PDB file : Tito_Scwrl_5AX7.pdb: