Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSTPEDISQNKPLLWLMAAACGLCAGVNYYCQPLIHSIQQDF--------AVTQAQAALTVTFAQVSYALGLLFIVPMGDIVNKAKAIPFLMMLCAVGLFTSA------------------FAVNLPMLWIGTILAGLFS-VAAQVLIPLATMTVKPEKTGEIIGFLMSGLLVGILLSTSLAGLFSNL--------FHWKVVYAISGILMLLLAYTLKSRLPYVMRM---KLNYGQI----------------FVSMAQ---LLKEEKRLLLRAL-----TG-----AFAFASVSILYSTIALLMTSAHHLPDLFIGLV-PLVGIFGALSTRYIGKYADQGYTRLLTWMGCGLFLLSWICFYFG---Q-TLLSAYVIGFALIQLALALVHTSNQSIIFR-LRPDAKSRINAIYMTTYFIGGAAGSALGI------FAWNRGEWTMTCLAGVGLVVFCILFSMIDAFLQKKQMA 4GBY Chain:A ((18-455)) -----------------TLGGLLFGYDTAVISGTVESLNTVFVAPQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGSAWPELGFTSINPDNTVPVYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIRGKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLML-LYTVPESPRWLMSRGKQEQAEGILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVGVIVIGVMLSIFQQFVGINVVLYYAPEVFK-TLGASTDIALLQTIIVGVINLTFTVLAIMTVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCWVLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGCMGVLA----------------

General information: TITO was launched using: RESULT: Template: 4GBY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1462 -219193 -149.93 -610.56 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -149.93 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.305

(partial model without unconserved sides chains): PDB file : Tito_4GBY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4GBY-query.scw PDB file : Tito_Scwrl_4GBY.pdb: