Modeling by threading (Tito software)
Unconserved sides chains calculation (Scwrl software)
Evaluation (QMean software)



Input alignment information:
Query sequenceMIGALMLDIAGTELTQEDIELLQAPQVGGMILFARNIESPQQVRALTDHMRQVRPDILIAVDQEGGRVQRLKSGFTLLPAMGRFGELYITQPQKALELAEQCGWLMATEVLAVGIDFSFAPVLDLN-AISDVIGDRGFSKNIEDIAPLAGAFMRGMKKAGMANTGKHFPGHGSVKADSHVAAAIDSRSYDEIYNHDMQSFIKLMPELDALMPAHVIYDQVDLNPAGFSPFWIQEVLRNRLKFNGVLFSDDLSMQAACVAGGADARIQAALAAGCDMGLVCNDRSAACTALDGITNLELPNQERLERMRGRIPQIQVGETLSLGNDWQAVKTAIEEFKNSF
5G2M Chain:B ((21-325))MQGSLMLDIGGTWLTAEDRQILRHPEVGGLIIFARNIEHPAQVRELCAAIRAIRPDLLLAVDQEGGRVQRLRQGFVRLPAM-----RAIADNPNAEELAEHCGWLMATEVQAVGLDLSFAPVLDLDHQRSAVVGSRAFEGDPERAALLAGAFIRGMHAAGMAATGKHFPGHGWA----HVAIPEDARSLEEIRRSDLVPFARLAGQLDALMPAHVIYPQVDPQPAGFSRRWLQEILRGELKFDGVIFSDDLSMAGAHVVGDAASRIEAALAAGCDMGLVCNDRASAELALAALQRLKVTPPSRLQRMRGK------------------------------


General information:
TITO was launched using:
RESULT:

Template: 5G2M.pdb
Alignment : align.pir
Tito was launched with SMD and SCWRL
Tito text output
Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1796 -103819 -57.81 -346.06
target 2D structure prediction score : 0.63
Monomeric hydrophicity matching model chain B : 0.85

3D Compatibility (PKB) : -57.81
2D Compatibility (Sec. Struct. Predict.) : 0.63
1D Compatibility (Hydrophobicity) : 0.85
QMean score : 0.456

(partial model without unconserved sides chains):
PDB file : Tito_5G2M.pdb:





(Unconserved sides chains are recalculated) :
Sequence: align-5G2M-query.scw
PDB file : Tito_Scwrl_5G2M.pdb: