Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKITTAIVLSMSGLIATTA-------MAAPHDHYHHQGYETKTVKVIHKDDHANIWRAGQVVPHQYQHPRYVIDYRSHQKLTKPGRYQKWYKVNGNYVLVNEREFGLSRI 4M20 Chain:B ((51-102)) -----TACSLGSANLIDTTKFIPLGLEMNANHIHSAKDGRVTATAEIIHRGKSTHVW------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4M20.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 53 -3043 -57.41 -67.61 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain B : 0.62 3D Compatibility (PKB) : -57.41 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.103

(partial model without unconserved sides chains): PDB file : Tito_4M20.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4M20-query.scw PDB file : Tito_Scwrl_4M20.pdb: