Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKRIARIAVATYMGLAVNMG-LAVNAVQAAT-TFLNVSYDPTREFYQ-EYNQAFGKFWKQRTGQDVDFKQSHGGSGKQARAVATGLEADVVTLALANDIDEIVKAGFIQPNWQKEFPNN-SAPYTSTVVFLVRKGNPKNI-RDWNDLTKPGVEIITPNPKTGGAPRWIYLSAWGYALKQPGGNDAKAKELVKKLYHNVNVLDLGARGSLTTFAERGIGDVLLSWENEALLATKGLDKDKYEIVYPSISILAEPSVAIVDKTVDKDGNRTLAKGYLNFLYSPLGQELAAKHYFRP-------RNPQVAAKY--AAQFPKIKLFTINDVFGGWAKAQKTHFANGAIFD-QIYDGKQ 5T1P Chain:A ((22-347)) -IDENLIKAAQAEGRVNSLAMPDTWANWKDTWADLKNLYDIEHSDTDMSSAQEIAKFKTEKKNASGD--IGDVGASFGEIAVKQG----VAQPFKTSYWDQI-------PTWAKDKDGNWLLAYTGTIAFIVNKDVVKDIPKTWQDLLKGNYKITVGDVSVA-AQAVSAVLAANYALGGDEKDLSPALAFFNTLAKQGRLVNNDVS---IANLEKGEVEVGLVWDFNGLGYRDKVGKDRYEVLIPADGSVISGYTTIINKYAK---HPNAAKLAREFILSDKGQINLAKGYARPIRIDHITLPDDIKAKLLPSEQYKNARAIKDQKAWEKSAKELPQLWQEKVIVDMK------

General information: TITO was launched using: RESULT: Template: 5T1P.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1823 97542 53.51 313.64 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 53.51 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.408

(partial model without unconserved sides chains): PDB file : Tito_5T1P.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5T1P-query.scw PDB file : Tito_Scwrl_5T1P.pdb: