Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAGNSIGQLFRVTTCGESHGVGLMAIVDGVPPGLALTEEDLQKDLDRRKPGTSKFATQRKEPDQVEIISGVFEGKTTGTPIGLLIRNTDQKSKDYGNIAQTFRPGHADYTYTQKYGFRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGILIRGHVTQIGNEVAEKLDWNEVPNNPFFCGDVDAVPRFEALVKSLREQGTSCGAKLEILA------EKVPVGWGEPVFDRLDADIAHAMMSINAVKGVEIGDGFAVAGQFGHETRDELTSHGFLANHAGGILGGISSGQTIRVAIALKPTASITTPGKTINLNREDTDVLTKGRHDPCVGVRATPIAEAMLAIVLMDHFLRHRAQNADVVPPFVPIEP 1UM0 Chain:C ((2-348)) ---NTLGRFLRLTTFGESHGDVIGGVLDGMPSGIKIDYALLENEMKRRQGGRNVFITPRKEDDKVEITSGVFEDFSTGTPIGFLIHNQRARSKDYDNIKNLFRPSHADFTYFHKYGIRDFRGGGRSSARESAIRVAAGAFAKMLLRE-IGIVCESGIIEIGGIKAKNYDFNHALKSEIFALDEEQEEAQKTAIQNAIKNHDSIGGVALIRARSIKTNQKLPIGLGQGLYAKLDAKIAEAMMGLNGVKAVEIGKGVESSLLKGSEYNDLMDQKGFLSNRSGGVLGGMSNGEEIIVRVHFKPTPSIFQPQRTIDINGNECECLLKGRHDPCIAIRGSVVCESLLALVLADMVL------------------

General information: TITO was launched using: RESULT: Template: 1UM0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1775 -77722 -43.79 -227.92 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain C : 0.83 3D Compatibility (PKB) : -43.79 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.404

(partial model without unconserved sides chains): PDB file : Tito_1UM0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1UM0-query.scw PDB file : Tito_Scwrl_1UM0.pdb: