Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLYQYHCACCDKVV-A-----STDKSCPYCGSHHIRSPYGLWMFCVMACLAVVVVFKVVHLYIQNHQDTPVQSTLLDVLNEGDKSTKQ 3WWL Chain:A ((2-33)) ---VGTCPECGAELRLENPELGELVVCEDCGAELE-----------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3WWL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 44 -2910 -66.14 -111.92 target 2D structure prediction score : 0.38 Monomeric hydrophicity matching model chain A : 0.48 3D Compatibility (PKB) : -66.14 2D Compatibility (Sec. Struct. Predict.) : 0.38 1D Compatibility (Hydrophobicity) : 0.48 QMean score : 0.851

(partial model without unconserved sides chains): PDB file : Tito_3WWL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3WWL-query.scw PDB file : Tito_Scwrl_3WWL.pdb: