Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSTQPMIEKLIEAHLDFLDEQFAQTQVIQQEFEQFYHWLGSRQLQHLWTFEQVQQLIQKQILDTPASDFLIEQIAEHIRFALIHPTNDTATVEDVIPVLTIDRIAQYVASKEEHRKKLIKTIVNNPAFSALLTQLIQQTMHDYLDESMTKRVPGVGRFMKMGKSVLETVTDSNLDDTINHYLQKNILKLSQMSKRVLNQHFDNDKLYHFQANVWHKVKTSPLSVLKNYIEVQDLTRTVGLGHEIWDHIRQTDYLKQQVHDGIYTWYVRNQERNFDLLLRDLNIDE-NLVKHELTELLVPVLQQLVTTGHLRRRARVYLEKFYYSEKALEILNNKGA 2L4M Chain:A ((110-156)) ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------EEDIYRFLKDNGPQRALVIAQALGMRTAKD--------VNRDLYRMKSRHLLDMD-----------------------

General information: TITO was launched using: RESULT: Template: 2L4M.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 126 -14061 -111.59 -305.66 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.54 3D Compatibility (PKB) : -111.59 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.54 QMean score : 0.658

(partial model without unconserved sides chains): PDB file : Tito_2L4M.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2L4M-query.scw PDB file : Tito_Scwrl_2L4M.pdb: