Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------------MTKKNNAPEL----HKYRGLT---STEQMVIHQMLISYVREE--------NCRFNIIM-TGTAEPYNLVKLTSINFENEASAIWVHFETITGEQIALPIDFLSRI-----EFSGQQEI-------------- 4B4P Chain:A ((6-147)) SAQVTGTLLGTGKTNTTQMPALYTWQHQIYNVNFIPSSSGTLTCQAGTILVWKNGRETQYALECRVSIHHSSGSINESQWGQQSQVGFGTACGN---KKCRFTGFEISLRIPPNAQTYPLSGDLKGSFSLTNKEVNWSASIYVP

General information: TITO was launched using: RESULT: Template: 4B4P.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 267 7110 26.63 75.64 target 2D structure prediction score : 0.38 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : 26.63 2D Compatibility (Sec. Struct. Predict.) : 0.38 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.096

(partial model without unconserved sides chains): PDB file : Tito_4B4P.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4B4P-query.scw PDB file : Tito_Scwrl_4B4P.pdb: