Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MITKTLPLTDIHRHLDGNIRIQTILELGQQYNLDLPAYDIESLRPHVQVMDNQPDLLSFLSKLDWGVKVLASL-DACKRIAFENMQDAAQQGLDYVELRFSPGYMGMAHQLPL-----------EGVVEAVIAGVKEGSQAYGVKANLIGIMSRTFGQEACEKELNALLAHKND--IKALDLAGDELGFPGNLFIDH---FKKARDAGWHITVHAGEAAGPESIWQAIEELGAERIGHGVKAVQDLKLLDYLAKHQIGIESCLTSNIQTNTVPSLAEHPLKTFLEHGVLATINTDDPAVEGIEIQHEYLTAAPLAGLSPEQIYTAQENGLKIAFLSEQEKDELRQKYQ 1A4M Chain:D ((7-332)) ------PKVELHVHLDGAIKPETILYFGKKRGIALPADTVEELR-NIIGMDKPLSLPGFLAKFDYYMPVIAGCREAIKRIAYEFVEMKAKEGVVYVEVRYSPHLLANSKVDPMPWNQTEGDVTPDDVVDLVNQGLQEGEQAFGIKVRSILCCMRH--QPSWSLEVLELCKKYNQKTVVAMDLAGDETIEGSSLFPGHVEAYEGAVKNGIHRTVHAGEVGSPEVVREAVDILKTERVGHGYHTIEDEALYNRLLKENMHFEVCPWSSYLTGAWDPKTTHAVVRFKNDKANYSLNTDDPLIFKSTLDTDYQMTKKDMGFTEEEFKRLNINAAKSSFL-------------

General information: TITO was launched using: RESULT: Template: 1A4M.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1824 31460 17.25 101.81 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain D : 0.75 3D Compatibility (PKB) : 17.25 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.396

(partial model without unconserved sides chains): PDB file : Tito_1A4M.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1A4M-query.scw PDB file : Tito_Scwrl_1A4M.pdb: