Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNILDEVFEQYSHELMDPNKAKATAKKIDKVY------EEEPFAALAIYLYAASQSWKKIYQPLKIPRTIYLATMNAFTRF-IHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYTDLRLEGKQCLSLHIPSDADLSQN----KIRDSYESAIRFFELYF--PDYHYQVMYTDTWLLSPNLTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQLLAGEHIGSGLGILTS 3RFT Chain:A ((1-266)) ---MAMKRLLVTGAAGQLGRVMRERLAPMAEILRLADLSPLDPAGPNEECVQCDLADANAVNAMVAGCDGIVHLGGISVEKPFEQILQGNIIGLYNLYEAARAHGQPRIVFASSNHTIGYYPQTERLGPDVPARPDG-------------LYGVSKCFGENLARMYFDKF--GQETALVRIGSCTPEPNNYRMLSTWFSHDDFVSLIEAVFRAPVLGCPVVWG----ASANDAGWWDN--------SHLGFLGWKPKDN------AEAFRRHITETTPPPD-PNDALVRFQGGTFVDNPIFKQ--

General information: TITO was launched using: RESULT: Template: 3RFT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1401 -331 -0.24 -1.31 target 2D structure prediction score : 0.39 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -0.24 2D Compatibility (Sec. Struct. Predict.) : 0.39 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.263

(partial model without unconserved sides chains): PDB file : Tito_3RFT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3RFT-query.scw PDB file : Tito_Scwrl_3RFT.pdb: