Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------MTEKGVETMKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKKYLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGALL-AAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGAN-AENKPEHLLQYGILGSFYARKVRGIGRPRVALLNNGTEATKG-SEVTKKAYELLQNETSLNFIGNVEARELLNGVADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAML---LKDGLRSLKAEMDYSK-HGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE 3TNG Chain:A ((5-291)) TKSRFFSDVAETSSFVFAVAGADDEVVLETIRLALKQKL-----GKFLLFGKKEDKTL--TANESVTWIQTDT---------------AEAAAQGAILAVKNKEADILVKGFIPTATLMHH---VLKKENGLRTDQLLSQIAIFDIPTYHKPLLITDCAMNVAPKTKEKIAITENALAVAHQIGITNPKIALLSAVEEVTAKMP-------------------STLEAQEVVQHFGNQISVSGPLALDVAISKEAALHKG--------ITDSSAGEADILIAPNIETGNALYKSLVYFAGAKVGSAVVGAKVPIVISSRNDSPENKLASFILTVRLVEK---------------

General information: TITO was launched using: RESULT: Template: 3TNG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1492 107559 72.09 392.55 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 72.09 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.499

(partial model without unconserved sides chains): PDB file : Tito_3TNG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3TNG-query.scw PDB file : Tito_Scwrl_3TNG.pdb: