Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------------MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEAGDEGVTPPV--DPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAAADVMND------GSRK-----PTYVQVTDRRSTLSGW-KLSVSQPEQFK---------TASG-DELVGAQ-LKFT-KGQAVSLVDPTYTPQTVNSELTL--TPGGNNTLAIN---AKSGTGVGTWVYRFGANENENQDAVQLSVPGKSVKLAQQYSTK-LVWTLEDTPNN------------------------------------------------------------------------ 5H5V Chain:A ((8-375)) LSAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHLAQAQTLTTRTTRDDTKTAIATSD-------SKLTIQQGGDKDPITIDISAANSSLSGIRDAINNAKAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEVSVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIKDWVNAYNSLIDTFSSLTKALLGDSTLRTIQTQLKSMLSNTVSSSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGSNLTSWLSTTGIIKAATDGV

General information: TITO was launched using: RESULT: Template: 5H5V.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1080 103955 96.25 440.49 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 96.25 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.230

(partial model without unconserved sides chains): PDB file : Tito_5H5V.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5H5V-query.scw PDB file : Tito_Scwrl_5H5V.pdb: