Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPITSENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKNEIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQTEKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKP-DQINARNVILANVQNDQKTYINQVVIETKITKKGHSEVLYQEEKEG-LQIAPNTNFSFPTALNGQPLTPGEYHLTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNNWIYILVASLFLLFILLCILLFVLRKKEEKEK 1G0D Chain:A ((481-666)) ----------------------------------------------------------QPVFGTDFDVIVEVKNEGGRDAHAQLTMLAM------AVTYNSLR----------RGECQRKTISVTVPAHKAHKEVMRLHYDDYVRCVSEHHLIRVKALLDAPG-------------PIMTVANIPL--S----TPELLV-QVPGKAVVWEPLTAYVSFTNPLPVPLKGGVFTLEGAGL-----LSATQIHVNGAVAPSGKVSVKLSFS--PMRTGVRKLLVDFD-----------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1G0D.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 807 -49865 -61.79 -275.49 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -61.79 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.365

(partial model without unconserved sides chains): PDB file : Tito_1G0D.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1G0D-query.scw PDB file : Tito_Scwrl_1G0D.pdb: