Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAVVGIPVGIMISRRR--KLANWVIRLANIIQTIPSLAMISILIIGLGL--GV-----------------------NVVIVTVFLYSLLPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIGITAI-GAFVGAGG-------LGDIIIRGTNA-TEGTSIILAGALPTALMAIITDWLLGILERRLDPASRTSR 4TQU Chain:M ((66-297)) --------------------SEQFIRAIKNTLTLSGLSLLFGFPMPILLALMINEVYSKGYRKAVQTIVYLPHFISIVIVAGLVVTFLSPSTGVVNNMLSWIGLDRVYFLTQPEWFRPIYISSNIWKEAGFDSIVYLAAIMSINPALYESAQVDGATRWQMITRITLPCIVPTIAVLLVIRLGHILEVGFEYIILLYQPTTYETADVISTYIYRLGLQGARY-DIATAAGIFNAVVALVIVLFANHMSRRITK------

General information: TITO was launched using: RESULT: Template: 4TQU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain M - contact count / total energy / energy per contact / energy per residue : 703 -97822 -139.15 -499.09 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain M : 0.65 3D Compatibility (PKB) : -139.15 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.216

(partial model without unconserved sides chains): PDB file : Tito_4TQU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4TQU-query.scw PDB file : Tito_Scwrl_4TQU.pdb: