Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSLTDILVCLSEKQNGRRNNMSDIIIGILLVISFFFMVWY--CVKGYNLMVGFAIMATVWMGL-------ALVGNTFSPNPAMEGQGVIDVLTHIYTTGPA---EYAKSILVNVFFGAFFGRVLVDS-------GIAATLIRKVVEL----GGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLP--------ILMSLGISVPVALFSFMGSIMAG----------------IFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVANISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIIL--GFILAGIWALLFTGKLRGGYKEICRQFAKLFTDGAVDVAP--MVGFLMTLAMFNNSAAYASPYFSAIFGDWIPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITTIAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA 4OV8 Chain:A ((6-472)) DTLNDVIQDPTRRNKLINDN--NLLKGIIMGRDGPVPSSRELIVRPDTLRAIINNRATIETTTMEAEFTETLMESNYNSASVKVSAPCITANSEYSESSSFKNTETEKSMYTSSRYLFPQGRIDFTTPDSGDVIKLSPQFTSGVQAALAKATGTEKREALQNLFQEYGCVFRTKVHIGGVLSAHTMETFSRSENETEVKQDVKAGLEGAVKGWGGGATAGHGNTQGTITTSQNRKLNVKYIVNGGDYTKI-QNTEEWVASTNQSEHW------RVIEVTEVTAVADL-LPQPIRGQVKDLLK------PLLGKWVDVEKVPGLESLPVSVYRPKGAIPAGWFWL---GDTADASKALLVKPTLPARSGRNPALTSLHQGSGMTEQPFVDLPQY--QYLSTYFGSFAHDTPP--GSTLRGLRPDHVLPGRYEMHGDT--ISTAVYVTRPVDVPFPEDEAFDLKSLVRVKLP--------GSGNPP-KPRSALKKSM---------------VLFD--

General information: TITO was launched using: RESULT: Template: 4OV8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2039 -112830 -55.34 -272.54 target 2D structure prediction score : 0.29 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -55.34 2D Compatibility (Sec. Struct. Predict.) : 0.29 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.153

(partial model without unconserved sides chains): PDB file : Tito_4OV8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4OV8-query.scw PDB file : Tito_Scwrl_4OV8.pdb: