Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----------------------------------------------------------------------------------------------------------------------MAIFVLGIYVTQLVIKNK----FLYFFAFSKTEYAKIRGYIATLNKR---------------------------- 2W69 Chain:A ((7-203)) AMEDFVRQCFNPMIVELAEKAMKEYGEDLKIETNKFAAICTHLEVCFMYSDFHFINEQGESIVVELDDPNALKHRFEIIEGRDRTMAWTVVNSICNTTGAEKPKFLPDLYDYKENRFIEIGVTRREVHIYYLEKANKIKSTHIHIFSFTGEEMATKADYTLDEESRARIKTRLFTIRQEMANRGLWDSFRQSER

General information: TITO was launched using: RESULT: Template: 2W69.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 124 -151 -1.22 -3.51 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.55 3D Compatibility (PKB) : -1.22 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.55 QMean score : 0.199

(partial model without unconserved sides chains): PDB file : Tito_2W69.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2W69-query.scw PDB file : Tito_Scwrl_2W69.pdb: