Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MISKRKHVIIFPILCKKIQGGGSTMIEEYIEKNILRQLFLCGQFYV-NKEVNLEKLSNLLHVCKTTLLNDINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDEPDYLQLTECEFISVSKAYSLKKQVLAYFN-DCGIEIDR--YSPRFTEMERRLLLLN-VSYRLGGFNSWELPESFFERADRFIESVTE-NSGRFYDKENKEILSIGFAISFLRQQVCAVTIDSKFIEEIK---KRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDFQQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFM------P--EKFYLLNEEQTNLYKEVQTIFSSWSSQLPYDLR--LNPNCMRAFVIE---LSGILRLTKEHLTIYIVTNSDVHYL-IYREALEA-VTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDAVQYENIIPISINTIDRAIISAVQNK 5WAY Chain:A ((1-480)) -------------------------MRDLLSKKSHRQLELLELLFEHKRWFHRSELAELLNCTERAVKDDLSHVKSAFPDL--IFHSSTNGIRIINTDDSDIEMVYHHFFKHSTHFSILEFIFFNEGCQAESICKEFYISSSSLYRIISQINKVIKRQFQFEVSLTPVQIIGNERDIRYFFAQYFSEKYYFLEWPFENFSS-EPLSQLLELVYKETSFP-MNLSTHRMLKLLLVTNLYRIKFGHFMEV--------FLMQAEGIEGVAQSFESE-YNISLDEEVVCQLFVSYFQKMFFIDESLFMKCVK-K-DSYVEKSYHLLSDFIDQISVKYQIEIENKDNLIWHLHN--TAHLYRQELFTEFILFDQKGNTIRNFQNIFPKFVSDVKKELSHYLETLEVCSSSMMVNHLSYTFITHTKHLVINLLQNQPKLKVLVMSNFDQYHAKFVAETLSYYCSN-NFELEVWTELELSK-ESLEDSPYDIIISNFIIPPI---ENKRLIYSNNINTVSLI-------

General information: TITO was launched using: RESULT: Template: 5WAY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1813 -31601 -17.43 -71.01 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -17.43 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.387

(partial model without unconserved sides chains): PDB file : Tito_5WAY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5WAY-query.scw PDB file : Tito_Scwrl_5WAY.pdb: