Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSVLVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFLCFYLPPKIFTLLGIQCLSFSFISYI-IVLIFLSFIVWMFYLVKK---- 4GKF Chain:A ((15-160)) ----RKTLEQRRGEYAYYVIKEVADLNDKQLEEKYASLVKKAPVMILSNGLLQTLAFLLAKAETSPEKANQILSRVNEYPPRFIEKLGNDKDEHLLLYLHIVYWLRENVDRNIDVKTLLSQDYSKVLWATKEAIALLNWMRRFAVAMLKE

General information: TITO was launched using: RESULT: Template: 4GKF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 589 -115356 -195.85 -818.13 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -195.85 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.383

(partial model without unconserved sides chains): PDB file : Tito_4GKF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4GKF-query.scw PDB file : Tito_Scwrl_4GKF.pdb: