Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVDMLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIAEMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKISESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKEIDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPAASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYPIDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGIAYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKLVSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLDGT-IEMNPRNEIVTEKNGATNIPGVFAAGDCTDS--PYKQIIVSMGSGATAALGAFDYLMRT 3F8R Chain:B ((14-316)) ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------EKFDVIIVGLGPAAYGAALYSARYMLKTLVIGETPGGQLTEAGIVDDYLGLIEIQASDMIKVFNKHIEKYEVPVLLDIVEKIENR-DEFVVKTKRKG-EFKADSVILGIGVKRRKLGVPGEQEFAGRGISYCSVCDAPLFKNRVVAVIGGGDSALEGAEILSSYSTKVYLIHRRDTFKAQPIYVETVKKKPNVEFVLNSVVKEIKGDKVVKQVVVENLKTGEIKELNVNGVFIEIGFDPPTDFAKSNGIETDTNGYIKVDEWMRTSVPGVFAAGDCTSAWLGFRQVITAVAQGAVAATSAYRYV---

General information: TITO was launched using: RESULT: Template: 3F8R.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1792 -172447 -96.23 -576.75 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain B : 0.67 3D Compatibility (PKB) : -96.23 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.490

(partial model without unconserved sides chains): PDB file : Tito_3F8R.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3F8R-query.scw PDB file : Tito_Scwrl_3F8R.pdb: