Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGRKTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSRIFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKWERVKNRVASKEE 3JWC Chain:B ((12-166)) ----MVAMDENRVIGKDNNLPWRLPSELQYVKKTTMGHPLIMGRKNYEAIGR-PLPGRRNIIVTRNEGYHV-EGCEVAHSVEEVFELCKN-EEEIFIFGGAQIFDLFLPYVDKLYITKIHHAFEGDTFFPEMDMTNWKEVFVEKGLTDEKNPYTYYYHVYEK----------

General information: TITO was launched using: RESULT: Template: 3JWC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 677 -92336 -136.39 -595.72 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain B : 0.81 3D Compatibility (PKB) : -136.39 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.348

(partial model without unconserved sides chains): PDB file : Tito_3JWC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3JWC-query.scw PDB file : Tito_Scwrl_3JWC.pdb: