Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDVRKSLQTILLKEAISMKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKVTASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSARLDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDTLLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQLGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANGNVTLKTWDS-NDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRV----------RADLVFYLPKRVYDHAAIKLLNGNIMIET-LEAKDIYTKSTN-GNIIVDQ-LTATMLEVEG-VNGNIDIRNGNILDSIIET-VNGTVTFGATAENLSVSL-VNGDVRLTIKEDNLKKVEAS-SVNGNVKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQLSTTTGSIYLKDTDK 3JX8 Chain:A ((19-232)) --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KISDYDEITFVG-SADFEYEQSDKAPYLSVTIDEN------------LFDYLVTEVEGGTLKIYPKSIKKGFNNNSYDLRPTVYKIKSNSKELKELNTV-GSGSFIISKPTKVNRMEINMAGSGNVELRGPVKGYKLECNMAGSGNIIAKDIQLDNLSCSLASSGEIEVIGTVDRASFNVAGSGEIKAFDCQ--ARKAECNIASSGEISVYATQIL--DANI-VGSGEIHYKG----------------------------------------------

General information: TITO was launched using: RESULT: Template: 3JX8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1142 51038 44.69 260.40 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 44.69 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.421

(partial model without unconserved sides chains): PDB file : Tito_3JX8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3JX8-query.scw PDB file : Tito_Scwrl_3JX8.pdb: