Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEVSRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGLYRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCLVAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK 1O5O Chain:C ((13-221)) MKNLVVVDHPLIKHKLTIMRDKNTGPKEFRELLREITLLLAYEATRHLKCEEVEVETPITKTIGYRINDKDIVVVPILRAGLVMADGILELLPNASVGHIGIYRDPETLQAVEYYAKLPPLNDDKEVFLLDPMLATGVSSIKAIEILKENGAKKITLVALIAAPEGVEAVEKKYEDVKIYVAALDERLNDHGYIIPGLGDAGDRLFRTK

General information: TITO was launched using: RESULT: Template: 1O5O.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1107 -178274 -161.04 -852.99 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain C : 0.89 3D Compatibility (PKB) : -161.04 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.89 QMean score : 0.555

(partial model without unconserved sides chains): PDB file : Tito_1O5O.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1O5O-query.scw PDB file : Tito_Scwrl_1O5O.pdb: