Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVNLADHLFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMI----KKMGNSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEVYLVRTLQ 2V34 Chain:B ((9-246)) ----------SPAKINLGLWVLGRLPSGYHEILTLYQEIPFYDEIY---IREGVLRVETNIG-IP-QEENLVYKGLREFERITGIEINYSIFIQKNIPPGAGLGGGSSNLAVVLKKVNELLGSPLSEEELRELVGSISADAPFFLLGKSAIGRGKGEVLEPVETEISGKITLVIPQVSSSTGRVYSSLREE-------HFVTPEYAEEKIQRIISGEVEEIENVLGDIARELYPEINEVY-RFVEYLGFKPFVSGSGSTVY------------------------------

General information: TITO was launched using: RESULT: Template: 2V34.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1198 -89362 -74.59 -381.89 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain B : 0.75 3D Compatibility (PKB) : -74.59 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.347

(partial model without unconserved sides chains): PDB file : Tito_2V34.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2V34-query.scw PDB file : Tito_Scwrl_2V34.pdb: