Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNYRVAVLGPGSWGTALAQVLAEN-------GHDVRIW-------GHRASQVEEINTQHTNQRYLPDHCLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVIIHASKGLEQGTH--KRISDILLEEIPVEKRKAVVVLSGPSHAEEVAKHDITTITAASTDAKAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMTRGLAEI---SRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQ-GQPLEEVLANM--GMVVEGVNTTQAAMELAQIMEI-----EMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKENEFSLTIF 2PLA Chain:B ((7-338)) ---KVCIVGSGNWGSAVAKIIGNNVKKLQKFASTVKMWVFEETVNGRKLTDI--INNDHENVKYLPGHKLPENVVAMSNLSEAVQDADLLVFVIPHQFIHRICDEITGRVPKKALGITLIKGIDEGPEGLKLISDIIREKMGID----ISVLMGANIANEVAAEKFCETTIGSKVMENGLLFKELLQTPNFRITVVDDADTVELCGALKNIVAVGAGFCDGLRCGDNTKAAVIRLGLMEMIAFARIFCKGQVSTATFLESCGVADLITTCYG--GRNRRVAEAFARTGKTIEELEKEMLNGQKLQG---PQTSAEVYRILKQKGLLDKFPLFTAVYQICYESRPVQ----------------------

General information: TITO was launched using: RESULT: Template: 2PLA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1732 -28391 -16.39 -93.08 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain B : 0.79 3D Compatibility (PKB) : -16.39 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.500

(partial model without unconserved sides chains): PDB file : Tito_2PLA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2PLA-query.scw PDB file : Tito_Scwrl_2PLA.pdb: