Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQRDLEKANITIL-PFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFIQNYTSIAVTGSHGKTSTTGLLSHVLS--GVRPTSYLIGDGTG---H---GDPQAEFFSFEACEYRRHFLAYSPDYAIMTNIDFDHPDYYT-SIDDVFTAFQTMAGQVK--KAIFAYGDDAYLRKLKANV--PIYYYGVTE-NDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVIAVAY-FEKLDLKEVAEEMLTFPGVKRRFSEKIV---------AD-MTVVDDYAHHPAEIKATIDGARQKYPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQG-NVKIEDLGAKIKKGG----EVI-KENNVSPLL---DYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL 2F00 Chain:B ((15-482)) EMRRVRHIHFVGIGGAGMGGIAEVLANEGYQISGSDLAP-NPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEARIPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDP-TFVNGGLVKAAGVHARLGH-GRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFLHNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADV-RVEDYQQIGPQGHFTLLRQDKEPMRVTLNAPGRHNALNAAAAVAVATEEG-IDDEAILRALESFQGTGRRFDFLGEFPLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYDDFANVLTQVDTLLMLEVYP-AGEAPIPGADSRSLCRTIRGRGKIDPILVPDPARVAEMLAPVLTGNDLILVQGAGNIGKIARSLAEIKLKP-----

General information: TITO was launched using: RESULT: Template: 2F00.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2479 -24640 -9.94 -56.91 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain B : 0.76 3D Compatibility (PKB) : -9.94 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.482

(partial model without unconserved sides chains): PDB file : Tito_2F00.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2F00-query.scw PDB file : Tito_Scwrl_2F00.pdb: