Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGYRHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQTDYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAKTAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHDKTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAKDPDTTQF 4FZI Chain:B ((13-283)) ----YNCVTLHNSVRMPQLGLGVWRAQDGAETANAVRWAIEAGYRHIDTAYIYSNERGVGQGIRESGVPREEVWVTTKVWNSDQGYEKTLAAFERSRELLGLEYIDLYLIHWPGKKKF--------VDTWKALEKLYEEKKVRAIGVSNFEPHHLTELFKSCKIRPMVNQVELHPLFQQRTLREFCKQHNIAITAWSPLGS--ILKNHVLGEIAKKHNKSPAQVVIRWDIQHGIVTIPKSTNKGRIQENFNVWDFKLTEEEMRQIDELNEDKRIGADPDN---

General information: TITO was launched using: RESULT: Template: 4FZI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1493 -133626 -89.50 -502.35 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain B : 0.83 3D Compatibility (PKB) : -89.50 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.531

(partial model without unconserved sides chains): PDB file : Tito_4FZI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4FZI-query.scw PDB file : Tito_Scwrl_4FZI.pdb: