Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MILVNIEGRLEKDDGTEEKKGIQKEVTIDRKANLKSHRLYWIGRRAQDVFCSALALIILSPVMLITALAIVIDDLGGSPIFAQNKVSRNGRLFKFYKFCSMCVDAEAKLDALLSQNEMDGPVFKIKNDPRITRVGKFI- 2DIP Chain:A ((1-98)) ----GSSGSSGLEEFKNSSKLVAAAEKERLDKHLG----------------------------------IPCNNCKQFPIEGKCYKCTE---CIEYHLCQECFDSYCHLSHTFTFREKRNQKWRSLEKRADEVSGPSSG

General information: TITO was launched using: RESULT: Template: 2DIP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 267 -8555 -32.04 -88.20 target 2D structure prediction score : 0.45 Monomeric hydrophicity matching model chain A : 0.53 3D Compatibility (PKB) : -32.04 2D Compatibility (Sec. Struct. Predict.) : 0.45 1D Compatibility (Hydrophobicity) : 0.53 QMean score : 0.215

(partial model without unconserved sides chains): PDB file : Tito_2DIP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2DIP-query.scw PDB file : Tito_Scwrl_2DIP.pdb: