Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEEVKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQ-IHVK---GTMVIAEAGASLIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLE-DGTLKTLTKDEMVFSYRHSKVQELR--AIVLEARFSLQTGDY-------------EA-I-----KARMDELTELRQSKQP--LEYPSCGSVFKRPVG--------------------------HYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAHIQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN 4JAY Chain:C ((4-340)) ------------LELQEHCSLKPYNTFGIDVRARLLAHARDEADVREALALARERGLPLLVIGGGSNLLLTR-DVEALVLRMASQGRRIVSDAADSVLVEAEAGEAWDPFVQWSLERGLAGLENLSLIPGTVGAAPMQNIGAYGVELKDVFDSLTALDRQDGTLREFDRQACRFGYRDSLFKQEPDRWLILRVRLRLTRRERLHLDYGPVRQRLEEEGIASPTARDVSRVICAIRREKLPDPAVLGNAGSFFKNPLVDATQAERLRQAFPDLVGYPQADGRLKLAAGWLIDKGGWKGFRDGPVGVHAQQALVLVNHGGATGAQVRALAERIQEDVRRRFGVELEPEPNLY---------------

General information: TITO was launched using: RESULT: Template: 4JAY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1384 -53915 -38.96 -190.51 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain C : 0.74 3D Compatibility (PKB) : -38.96 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.516

(partial model without unconserved sides chains): PDB file : Tito_4JAY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4JAY-query.scw PDB file : Tito_Scwrl_4JAY.pdb: