Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MCTAITYATKD--HYFGRNFDYEMSYNEVVTITPRNYR-FDFRKVKNLDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADY-KELQEGKDNVAPFEFIPWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLAD-KEKSIVIESMKDGLHLYDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTRV 2RF8 Chain:B ((1-192)) MATGLALETKDGLHLFGRNMDIEYSFNQSIIFIPRNFKCVNKSNKKELTTKYAVLGMGTIFDDYPTFADGMNEKGLGCAGLNFPVYVSYSKEDIEGKTNIPVYNFLLWVLANFSSVEEVKEALKNANIVDIPISENIPNTTLHWMISDITGKSIVVEQTKEKLNVFDNNIGVLTNSPTFDWHVANLNQYVGL------

General information: TITO was launched using: RESULT: Template: 2RF8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 997 26228 26.31 140.25 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain B : 0.85 3D Compatibility (PKB) : 26.31 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.433

(partial model without unconserved sides chains): PDB file : Tito_2RF8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2RF8-query.scw PDB file : Tito_Scwrl_2RF8.pdb: