Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRTLIIISHPTIEES-LNQQFFKEASAGLPATWHHLESSYPDGKIDIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLE------RAAHVLKGKELGIVLTTGVAEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQQYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQEMKGTSL 3L9W Chain:B ((240-382)) ---LIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNIDIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLWAVTTGGGESHFEIGAHPGFDV--LSQPLQATAIYCGLNWLPPFAM------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3L9W.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 519 -17365 -33.46 -127.68 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain B : 0.70 3D Compatibility (PKB) : -33.46 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.677

(partial model without unconserved sides chains): PDB file : Tito_3L9W.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3L9W-query.scw PDB file : Tito_Scwrl_3L9W.pdb: