Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNGAEIFQGTYSINGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPIFNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKANFLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQSVLVGVYPFISSSS-----EGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDV-ATLREAAKDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSARIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDL-----LNDIVDHQTDMITGQNGGYVLL----DPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGINGTYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKDIMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFRYSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLARFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDNGKVRVDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRLGYEDIRLKEDTAYDSTVLDQKRF 5JM8 Chain:A ((11-574)) -------------TLDVAAQCFLNSLVRETKDWRLTEYQPT------------------QLIIP--------LGEQQALHFRVAYF-SPTQHHRFEFPARLVTASGSHPV--------------------------------DFATLSRLIVD-KLQHQLLLP------ATSCETFHQRVMESHAHT--QQAIDARHDWAALREKALNFGEAE---QALLVGHAFHPAPKSHEPFNQQEAERYLP------DFAPHFPLRWFAVNKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDNQWLFPL-HPWQGEYLLQQEWCQELVAKGLIKDLGEAGAPWLPTTSSRSLYCATSRDMIKFSLSVR-LTNSVRTLS------VKEVKRGMRLARLAQTDDWQTLQARFPTFRVMQEDGWAGLRDLHGNIMQESLFALRENLLVDQPQS---QTNVLVSLTQAAPDGG-------DSLLVAAVKRLSDRLGIT-------------AQQAAHAWVDAYCHQVLKPLFTAEADY-----------------GLVLLAHQQNILVQML--------------------------GDLPVGLIYRDCQGSAFMPHAAGWLD------------------------------------TIGEAQAENVFTREQLLRYF-----PYYLLVNSTFAVTAALGAAGLD-----SEANLMARVRTLLAEMRD---QVTHKTCLNYVLENP------YWNVKGNFFCYLNDPSVIYFDFANPLLAQ---

General information: TITO was launched using: RESULT: Template: 5JM8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 3078 -12059 -3.92 -22.29 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -3.92 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.244

(partial model without unconserved sides chains): PDB file : Tito_5JM8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5JM8-query.scw PDB file : Tito_Scwrl_5JM8.pdb: