Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRRLLPIKKLLGSTAGFRLASTKSSGTERLERLVEEVNGIKSKLKVLEESKAANGLVDLYASRKMKRLDIKRILSIFNDR-AYHAPIFCHKALPIILAHFITGLDKLPSGLNAMPSILAVRATLLRSFQKLINCKI--PATDDQVQHFRRVLEDIDEEHAERDLLQTMAFGILELKEYVSSHRRALVDLKKTSERWASIPMTEENVLTYAEIQDIQAPLDSVNRCMITYNFISRMFLNHDPDMTMGSNPRRIGMVDLEMNLEHVVRNAVDEAKQICTDHYGDCPDTEFEFTS---DSKAFRFPYMSTTIRYIILELMKNAFRATVDSHMKRNDVGMVTCADMPPVRVLINLQEGTEHACICISDEGMGMTDEALAMAMAYSYTSVSKPALQLGDSGEGCASTAPSPLAGYGYGLPMSRVYAQSLGGDLFLQTMEGYGTRAYYYIKIADAQPLCDEETK 5J71 Chain:A ((6-366)) -------------------------------------------------LAGAPKYIEHFSKFSPSPLSMKQFLD-----CEKTSFTFLRQELPVRLANIMKEINLLPDRVLSTPSVQLVQSWYVQSLLDIMEFLDKDPEDHRTLSQFTDALVTIRNRHND--VVPTMAQGVLEYKDTYGDD--------------------------PVSNQNIQYFLDRFYLSRIS----IRMLINQHTLIF------HIGSIDPNCNVSEVVKDAYDMAKLLCDKYYMASPDLEIQEINAANS-QPIHMVYVPSHLYHMLFELFKNAMRATVESHES--------SLILPPIKVMVA--LGEEDLSIKMSDRGGGVPLRKIERLFSYM-----------------------------GYGLPISRLYAKYFQGDLQLFSMEGFGTDAVIYLKALSTD--------

General information: TITO was launched using: RESULT: Template: 5J71.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1426 -28772 -20.18 -92.22 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -20.18 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.575

(partial model without unconserved sides chains): PDB file : Tito_5J71.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5J71-query.scw PDB file : Tito_Scwrl_5J71.pdb: