Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSRPCLLLLGIDVVFAHISGSCINHVGRADLVMLRRGFCRSIPLLGPTFTELVERLDSFEKEFIEWKAFRKAVESTSPKVTEAAKEIIAESMKEVKTSKVNLENPLTPEEFTELNQFYAKQKVKDIFFENLLYINTPNDLMQHSETVFRQYLVRIARRVRHLNHAPYGLSQMPGIQMLKKWYQWSFHDVRSTPV--PTTRDECYRCDRMVRRVFLRHYNVSSLITDGMVEFASREGWTHVDEEVMRTYDELQNFFEAFCLGRVRLRFLVGNYMYLSTKILGVSKEEYAVNDPDGLTVPIFFDHNAEDFVGQICKKCSLLVLTKCAIKEAQASYD------AEIELKLAG----DPNLVFVGIPYITYDIICAMLEDAVSANVDRQERTGKACTKIEVTLAQWPTNKRFVLRISDTAGGMTLRQASKQLSCWSLYRNIQGHNQDTISTWTSSPIRLPYAYNAARVIGGNITLASIEGYGTDRQLYLPSTGLAGVSL 5J71 Chain:A ((8-366)) -------------------------------------------------------------------------------------------------------------GAPKYIEHFSKFSPSPLSMKQFLD-----CEKTSFTFLRQELPVRLANIMKEINLLPDRVLSTPSVQLVQSWYVQSLLDIMEFLDKDPEDHRTLSQFTDALVTIRNRHNDVVPTMAQGVLEYKDTYGDD------PVSNQNIQYFLDRFYLSRISIRMLINQHTLIF-------------------------------HIGSIDPNCNVSEVVKDAYDMAKLLCDKYYMASPDLEIQEINAANS-QPIHMVYVPSHLYHMLFELFKNAMRATVESHES-SLILPPIKVMVA--LGEEDLSIKMSDRGGGVPLRKIERLFSYM--------------------GYGLPISRLYAKYFQGDLQLFSMEGFGTDAVIYLKALSTD----

General information: TITO was launched using: RESULT: Template: 5J71.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1305 -44748 -34.29 -147.20 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -34.29 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.495

(partial model without unconserved sides chains): PDB file : Tito_5J71.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5J71-query.scw PDB file : Tito_Scwrl_5J71.pdb: