Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTSTSVALIASVLRQPRVGDDLQWVRYDAPFRTAQDYAAAEYLYRLRLCSLRLRSSLLSPSLPRTGPVSEVAHLLPHYPVQITAQLVYMDEPLSPVVESAAVYASAAALLPPASPVGGDTDTHAEMEVVYTFTDEWLVFPLELCDVPLDASCKLHLWHR---------DTHVAEAALHVYSVAGELCVGQRQLALSTGGDAPSDEQLWSTSTRAAELLADFHRGMMPPIPWLDTLSIQQLEAEHARHGNAVVRRGSPQSAKDPCGAPTAILTLYLPAATT-AVFFEPGVARVSDDMKSLLQHSDAADDSCDFTQRPFPDQYTFFKEHNLCEAKAAITSKTQYFLSDSSAPPGPKERHQLASLLRRPPIQLDNVAGAVVTGAGAGGVGGGRLEETRLLWKYRHFICRDGKYFLPFMRCVDWANTHSSERRAACALIYQWARPAFEDVLACLSFYFDHVAPVRQYAVRLLRR-EGDGRLCQLAFQLVQAVRYDSAE-AELANFLVERAVGCWELCSTLYTLLSVEVALEKRRTLSAAGKASDDRHGGRALFEPLLRRLSERLTQQCPHFATRLRQQHAMHRVLQLLSRQLQQSSLDRLGKTALGNKLIAKQACGLRALFSSVHHGMPSRRNVNGSFSSSLMTHSSAGMREEHSMSSARTGDGSEEADANGGDGSSAVEASDVDSAADDEVRRLRFPPLSPQSQGSAPRSPPSQSGAKQQYRSRHASAVDVLDRYGVATLATHPGIPITGLLSDSLYIFKSAKLPMRLTFTALRPAGLAWGGGRGGEPLYGASPSPCLLTPLAMQPAPSQQRSGGLQGMNSSGGGASAAVAAEEQAEGFLGAGEGDTVPLAMMYKYNDDIRQDQLIVQLIRLMDDLLQRDGLQLYLTPYRVIATAPNEGLVEIVPQVTTFFSVQ-----RDVLKYLRVYNSTAELLRQAMDRYTRSFAGYCVITFVLGIGDRHLENILITQDGRLLHIDFGYVLGNDPKP-----FPPPMKINREMVEVLGGP-----QSTGFTEFKLYCCSAYNTLRKHAPLLLHILLLGAHTEGMPQVTGEGGDPRVNLLKVQEKLRLDLTNAQATQYLQNVIADSV-GSIFTNLWDVLHAAAQATRG 4JPS Chain:A ((350-1061)) ---------------------------------------------------------------------------DIDKIYVRTGIYHGGEPLCDNVNTQRVPCSN-----------------------P-RWNEWLNYDIYIPDLPRAARLCLSICSVKGRKGAKEEHCPLAWGNINLFDYTDTLVSGKMALNLWPVPHGLE------DL--------------LNPIGV--------------TGSN-------------P-NKETPCLELEFDWFSSVVKFPDMSVIEEHA------------N-------------------------------------------E--NDKEQLKAISTRDPLS-------EI-----------TEQEKDFLWSHRHYCVTIPEILPKLLLSVKWNS--RDEVAQMYCLVKDWPPIKPEQAMELLDCNYP-DPMVRGFAVRCLEKYLTDDKLSQYLIQLVQVLKYEQYLDNLLVRFLLKKALTNQRIGHFFFWHLKSEMHNKTV----------------SQRFGLLLESYCRACGM----YLKHLNRQVEAMEKLINLTDILKQEKKDETQKVQ-MKFLV----------------------------------------------------------------------------------EQMRRP---D--------------------------FMDA---LQGFLSPLNPAHQLGNLRLEECRIMSSAKRPLWLNWENPDIMS----------------------------------------------------------------ELLFQN--NEIIFKNGDDLRQDMLTLQIIRIMENIWQNQGLDLRMLPYGCLSIGDCVGLIEVVRNSHTIMQIQCKFNSHTLHQWLKDKNKG-EIYDAAIDLFTRSCAGYCVATFILGIGDRHNSNIMVKDDGQLFHIDFGHFLDHKKKKFGYKRERVPFVLTQDFLIVISKGAQECTKTREFERFQEMCYKAYLAIRQHANLFINLFSMMLGS-GMPELQSF-----DDIAYIRKTLALDKTEQEALEYFMKQMNDAHHGGWTTKMDWIFHT-------

General information: TITO was launched using: RESULT: Template: 4JPS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 3135 -9324 -2.97 -14.37 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -2.97 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.431

(partial model without unconserved sides chains): PDB file : Tito_4JPS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4JPS-query.scw PDB file : Tito_Scwrl_4JPS.pdb: