Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence GAKCRVDLKHGQAVVSSFEVLAG--SRKTVKEEGFSWTCSTERDTCNAVNCILPKGLTAVGVQISSGKPKDRAAEESAARQAAAPKVVA------------------ 1TOL Chain:A ((125-215)) ---------SGADINNYAGQIKSAIESKFYDASSY------AGKTCTLRIKLAPDG-MLLDIKPEGGDPALCQAALAAAKLAKIPKPPSQAVYEVFKNAPLDFKPAA

General information: TITO was launched using: RESULT: Template: 1TOL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 199 10598 53.25 149.26 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : 53.25 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.343

(partial model without unconserved sides chains): PDB file : Tito_1TOL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1TOL-query.scw PDB file : Tito_Scwrl_1TOL.pdb: