Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAKQTIIVMSDSHGDSLIVEEVRDRYVGK-VDAVFHNGD------------SELRP-----------DSPLWEGIR-----------------------VVKGNMDFYAGYPERLVTELGSTKIIQTH-------GHLFDINFNFQKLDYWAQEEEAAICLYGHLHVPSAWM-EGKILFLNPGSISQPRGTIR------ECLYARVEIDDSYFKVDFLTRDHEVYPGLSKEFSR-------------------------- 3QFM Chain:A ((11-268)) --MTKIALLSDIHGNTTALEAVLADARQLGVDEYWLLGDILMPGTGRRRILDLLDQLPITARVLGNWEDSLWHGVRKELDSTRPSQRYLLRQCQYVLEEISLEEIEVLHNQPLQIHRQFGDLTVGISHHLPDKNWGRELIHTGKQEEFDRLVTHPPCDIAVYGHIHQQLLRYGTGGQLIVNPGSIGQPFFLDAQLRKDLRAQYMILEFDDKGLVDMDFRRVDYDVAAELQLAKDLRLPYFEVYYESLVNGIHHTHHQEFL

General information: TITO was launched using: RESULT: Template: 3QFM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 765 8494 11.10 49.67 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : 11.10 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.249

(partial model without unconserved sides chains): PDB file : Tito_3QFM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3QFM-query.scw PDB file : Tito_Scwrl_3QFM.pdb: