Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTSLNSSYGRSQAVPLSVFEQSKDAHIIRSDEEAIEIAKQLAQEFATEASDRDRERRLPLNEIQQFSQSGLWAITVPKQFGGAQVKYRTLAEVIKTIASVDSSLAQLPQNHLAFIEHLRLDAKPEQQQFFFNLVLRGIRF-GNAFSEKHSKTVADLTTTLRKTEHG-YEINGKKFFATGALLAHWIPIVAVNELGQP-----YAALVPRETSGVNIINDWSGFGQRTTASGTVELNQVPVREEYLVPIYQAF-------ESPTPAGAISQFIQAAVDAGIARGAIEETIQYVKNHSRAWIDSGLEKASDDPYTIANIGDLKIKLRAA-----------EAVLDLAGDAIDQAIANTTEEHVNQATLLVAEAKVLTTEIAILAANKLFELSGTRSTLSELNLDRHWRNARTHTLHDPVRWKYHIVGNYYLNGVQPPRHAWS 4DOY Chain:E ((38-431)) -------------------------------NDPVAVARGLAEKWRATAVERDRAGGSATAEREDLRASGLLSLLVPREYGGWGADWPTAIEVVREIAAADGSLGHLFGYHLTNAPMIELIGSQEQEEHLYTQIAQNNWWTGNASSENNSH-VLDWKVSATPTEDGGYVLNGTKHFCSGAKGSDLLFVFGVVQDDSPQQGAIIAAAIPTSRAGVTPNDDWAAIGMRQTDSGSTDFHNVKVEPDEVLGAPNAFVLAFIQSERGSLFAPIAQLIFANVYLGIAHGALDAAREYTRTQARPWTPAGIQQATEDPYTIRSYGEFTIALQGADAAAREAAHLLQTVWD-KGDAL-------TPEDRGELMVKVSGVKALATNAALNISSGVFEVIGARGTHPRYGFDRFWRNVRTHSLHDPVSYKIADVGKHTLNGQYP------

General information: TITO was launched using: RESULT: Template: 4DOY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 1967 -22572 -11.48 -61.17 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain E : 0.76 3D Compatibility (PKB) : -11.48 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.411

(partial model without unconserved sides chains): PDB file : Tito_4DOY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4DOY-query.scw PDB file : Tito_Scwrl_4DOY.pdb: