Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNSSQEPNQSLFNQASTSIQACIECMFACKHCASACLQEEHVHMMRKCIKHCLTCVETCQLCTSLELRHSELAEQAMQLCANACQLCAAECSKHEHEHCQVCAKACLACAQACQAYRA 3KAW Chain:G ((31-130)) -----------QAPEERCRLAAQACIRACERYLALCTESS-----REQRQHAGDCADLCRLAALLLERRSPWAPAACELAARYALACAERCDGD-EPLERECAGACRRFVEACRPLL-

General information: TITO was launched using: RESULT: Template: 3KAW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain G - contact count / total energy / energy per contact / energy per residue : 462 -62513 -135.31 -625.13 target 2D structure prediction score : 0.78 Monomeric hydrophicity matching model chain G : 0.69 3D Compatibility (PKB) : -135.31 2D Compatibility (Sec. Struct. Predict.) : 0.78 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.632

(partial model without unconserved sides chains): PDB file : Tito_3KAW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3KAW-query.scw PDB file : Tito_Scwrl_3KAW.pdb: