Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEKLIITAAVNGGITPRSKNPAVPYTPEEIANAVYEVWNAGASVAHIHARNLDGSPSYQQEVWGEIVDKVRARC-DIILNLSTSGLNLPLDAPKDQAWNHLVYRPEIASYNCGSVNHGSKPFINPPALAMELADAINQYGVKPEIEIYHSGVINEAETLHLKGYL-KSPMLFAFAMGIHGGVTATCKNLI----HLIDSLPAGSLWSALGIGKAQLPINVHTILLGGHVRTGLEDNIYYKAGELATSNAQLVERLVRLSHELDRPVASTQEARKILGLVS 2Y7F Chain:B ((8-280)) -EPLILTAAITGAETTRADQPNLPITPEEQAKEAKACFEAGARVIHLHIREDDGRPSQRLDRFQEAISAIREVVPEIIIQISTGG---AVGESFDKRLAPLALKPEMATLNAGTLNFGDDIFINHPADIIRLAEAFKQYNVVPEVEVYESGMVDAVARLIKKGIITQNPLHIQFVLGVPGGMSGKPKNLMYMMEHLKEEIPTAT-WAVAGIGRWHIPTSLIAMVTGGHIRCGFEDNIFYHKGVIAESNAQLVARLARIAKEIGRPLATPEQAREILAL--

General information: TITO was launched using: RESULT: Template: 2Y7F.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1541 -49265 -31.97 -184.51 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain B : 0.81 3D Compatibility (PKB) : -31.97 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.478

(partial model without unconserved sides chains): PDB file : Tito_2Y7F.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2Y7F-query.scw PDB file : Tito_Scwrl_2Y7F.pdb: